Microcin E492 is an unmodified peptide related in structure to colicin V.
نویسندگان
چکیده
The pore-forming microcin E492 was purified by solid-phase extraction and reversed-phase high-pressure liquid chromatography. Its molecular mass was 7,886 Da. The entire 84-amino-acid sequence was determined. There is no postranslational modification in the secreted microcin, and the sequence has homologies with the sequence of the microcin colicin V.
منابع مشابه
Microcin E492, a low-molecular-weight peptide antibiotic which causes depolarization of the Escherichia coli cytoplasmic membrane.
Microcin E492 is a 5,000- to 7,000-molecular-weight peptide antibiotic which depolarizes the cytoplasmic membrane of sensitive Escherichia coli K-12 cells. The microcin has many features in common with colicin V but is distinguished from it by the absence of microcin E472 immunity in ColV+ strains and by the fact that colicin V-insensitive Cir mutants of E. coli K-12 are microcin sensitive. Nei...
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Microcin H47, a gene-encoded peptide antibiotic produced by a natural Escherichia coli strain, was shown to be secreted by a three-component ATP-binding cassette exporter which was revealed to be strongly related to that of colicin V. The results of sequence and gene fusion analyses, as well as heterologous complementation assays, are presented.
متن کاملMicrocin e492 amyloid formation is retarded by posttranslational modification.
Microcin E492, a channel-forming bacteriocin with the ability to form amyloid fibers, is exported as a mixture of two forms: unmodified (inactive) and posttranslationally modified at the C terminus with a salmochelin-like molecule, which is an essential modification for conferring antibacterial activity. During the stationary phase, the unmodified form accumulates because expression of the matu...
متن کاملThe production in vivo of microcin E492 with antibacterial activity depends on salmochelin and EntF.
Microcin E492 is a channel-forming bacteriocin that is found in two forms, namely, a posttranslationally modified form obtained by the covalent linkage of salmochelin-like molecules to serine 84 and an unmodified form. The production of modified microcin E492 requires the synthesis of enterochelin, which is subsequently glycosylated by MceC and converted into salmochelin. mceC mutants produced ...
متن کاملWhole-Genome Sequence of the Microcin E492-Producing Strain Klebsiella pneumoniae RYC492
Here, we report the draft genome sequence of the Gram-negative strain Klebsiella pneumoniae RYC492, which produces the amyloid-forming and antibacterial peptide microcin E492. The sequenced genome consists of a 5,095,761-bp assembled open chromosome where the gene cluster for microcin production is located in a putative 31-kb genomic island flanked by sequence repeats and containing a putative ...
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عنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 46 1 شماره
صفحات -
تاریخ انتشار 2002